Broad Clinical Research Sequencing Platform is now Broad Clinical Labs! Explore our new website to access our trusted services.
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Broad Clinical Research Sequencing Platform is now Broad Clinical Labs! Explore our new website to access our trusted services.
Access both research and clinical-grade whole genome sequencing services backed by 30 years of field-leading experience in providing high quality, reliable data at scale.
Our WGS services utilize Illumina’s latest technology, the NovaSeq X Plus. All samples for WGS services are processed in our CLIA licensed and CAP accredited facility with rigorous quality control throughout processing. We provide dedicated project management to guide you through project onboarding, sample logistics management, and data delivery.
We offer both PCR-free and PCR-plus Research WGS services. While PCR-free services yield superior coverage evenness and the lowest GC bias, PCR plus services enable high quality data generation from samples whose quality or quantity is limiting (e.g. FFPE).
We offer Clinical WGS services with either a technical report or an interpreted report, depending on your needs.
This long read output can be used for de novo sequence assembly, small and large variant detection, haplotype phasing, and epigenetic characterization (methylation).
| Services | Inputs | Outputs |
|---|---|---|
| PCR-Free WGS on Illumina® Platform (Research); 30x or 60x coverage | Genomic DNA (≥750ng, ≥7ng/µl), Whole Blood, Saliva, Buccal Swabs, PBMCs, Cell Pellet, Buffy Coat, Fresh Frozen Tissue, and more | Aggregated, aligned sequencing data (CRAM file); variant calling files (gVCF, individual VCF files), including SNVs, InDels, CNVs; joint calling also available |
| PCR-Plus WGS on Illumina® Platform (Research); 30x or 60x coverage | Genomic DNA (≥250ng, ≥2ng/µl) or FFPE tissue | Aggregated, aligned sequencing data (CRAM file); variant calling files (gVCF, individual VCF files), including SNVs, InDels, CNVs; joint calling also available |
| Long-read WGS on PacBio Platform (Research); 12x or 24x coverage | High Molecular Weight Genomic DNA (≥4µg, ≥30ng/µL, ≥100µL, with ≥50% DNA ≥40kB fragment size. Recommeded A260/230≥1.8) | CCS error-corrected, aggregated, and aligned BAM files |
| Clinical WGS (PCR-Free, Illumina® platform) | Genomic DNA (≥1µg, ≥10ng/µL), Whole Blood, Saliva, or Buccal Swab | Technical report, or clinical interpretive report (SNVs, InDels, CNVs) with variant prioritization and annotation; screening and diagnostic interpretive reports available; read and variant level data available for research use |
Regulatory Review
Quoting
Sample Kits
Sample types vary by platform and service
Clinical includes: whole blood, buccal swab, saliva
PCR-Free
PCR-Plus
PacBio CCS
Illumina (short read)
PacBio (long read)
See table above for available research and clinical service deliverables
Data delivered via secure
Cloud based platform
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