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Sequencing of
Customer-Prepared Libraries

Our rapid “Walk-Up Sequencing” (WUS) service generates and delivers data from your sequencing-ready libraries and library pools.

Take advantage of our sequencing capacity on Illumina® NovaSeq X Plus, PacBio Revio, and a variety of additional technologies.

Your libraries on a short-read sequencer — as quickly as

2 days

Please note: if pooling is needed, libraries must be pooled by the customer prior to receipt at Broad Clinical Labs.

NovaSeq X Plus starting as low as $13,000 per 10B flowcell

Illumina® NovaSeq X Plus

PacBio Revio™ long reads starting as low as $2,000 per SMRT™ Cell

PacBio Revio™

The newest of the Illumina® fleet, the NovaSeq X series includes the NovaSeq X Plus 10B (available now) and the NovaSeq X Plus 25B (coming soon). With faster run times, higher outputs, increased reads per run, and new reagent methodologies, the new X Series produces cost-effective, fast sequencing with deeper coverage. NovaSeq X Plus Series offers the flexibility to submit multiple pooled libraries to run within one flowcell, or alternatively to run one library on multiple lanes in a flowcell to yield the maximum amount of coverage per library. These extremely cost-effective machines are best suited for large scale applications with a 300 cycle modality (2x150bp) for studies such as high volume whole genome, whole exome, or whole transcriptome sequencing.

The Revio™ is the latest, highest output PacBio sequencing system. Long Read sequencing with the Revio™ is suitable for a variety of applications that benefit from long reads, including but not limited to the following: more comprehensive profiling of variants in human whole genomes; full isoform sequencing in single cell and bulk transcriptomes. A single SMRT™ Cell is expected to produce ≥4 million reads.

Producing ≥5 million reads per flowcell, the MiSeq is recommended for small whole genome sequencing (microbe, virus), targeted gene sequencing (amplicon-based or gene panels), and 16S metagenomic sequencing, but can also be used for targeted gene expression profiling, microRNA and small RNA sequencing, and DNA-protein interaction (ChIP-Seq).

Producing ≥320 million reads per flowcell, the NextSeq 500 is recommended for low scale or small whole genome sequencing, targeted sequencing (amplicon or panel-based sequencing), and higher throughput transcriptome sequencing such as total RNA-Seq, mRNA-Seq and gene expression profiling. It is also suitable for exome sequencing, ChIP-Seq, methylation sequencing, metagenomics including 16S, and transcriptome sequencing for single-cell profiling, targeted gene expression studies, and miRNA and small RNA sequencing.

Producing ≥800 million reads per flowcell on the NextSeq 2000 P3 flowcell (≥320M for P2), the faster turnaround time of this mid-throughput technology is suitable for a wide variety of studies including: low-throughput whole genome, exome, and transcriptome sequencing; targeted applications such as amplicon sequencing or gene panels of various sizes; a wide variety of transcriptome applications including both single-cell and bulk transcriptome studies; microbiome studies that may need metagenomic, metatranscriptomic, or 16S profiling.

With a wide variety of modalities (see below), the NovaSeq 6000 Series offers high-throughput processing that generates consistent data at scale for a variety of studies. With variable loading per lane, the NovaSeq 6000 Series offers the flexibility to submit multiple pooled libraries to run within one flowcell, or alternatively to run one library on multiple lanes in a flowcell to yield the maximum amount of coverage per library. These machines are typically utilized for high-throughput studies such as large scale whole genome, whole exome, or whole transcriptome sequencing.

With independent dual flowcells and the flexibility of 150 or 300 cycle runs, the Element AVITI™ System utilizes a novel rolling circle amplification technology to reduce PCR error propagation, AT/GC bias, and provide accurate sequencing data for a wide variety of applications. With typical runs yielding ~600GB of data, this mid-throughput machine is suitable for a variety of cost-effective studies such as exome, low-pass genome, and bulk and single-cell RNA sequencing. Compatible with linear libraries generated for Illumina® machines, the Element Adept Library Compatibility Workflow can adapt libraries prepared with third-party kits for use on the Element AVITI™ System, producing circularized libraries in <2 hours.

Specifications

See Walk-up Sequencing data sheet for more information.

TechnologyMinimum orderMinimum library volumeMinimum library concentrationDeliverable
Illumina® MiSeq, NextSeq 500, NextSeq 2000Flowcell20μL2nMDemultiplexed FASTQ or BCL files
Illumina® NovaSeq 6000 (multiple flowcell types)Flowcell140μL for S4 flowcell; 70μL for other flowcell types2nMDemultiplexed FASTQ or BCL files
Illumina® NovaSeq X Plus 10BFlowcell200μL2nMDemultiplexed FASTQ or BCL files
PacBio RevioSMRT™ Cell30μL20—60 ng/μL (Concentration varies based on library fragment size)Circular consensus error corrected, aggregated, and aligned output file
Element AVITIFlowcell70μL6nMDemultiplexed FASTQ files

Sequencing with Broad Clinical Labs

Project onboarding

Project onboarding

Library Receipt at BCL

Library Receipt at BCL

Library QC

Library QC

Sequencing

Sequencing

Illumina® (short read)
PacBio (long read)
Element (short read)

Data delivery

Data delivery

Outputs:

De-multiplexed FASTQs
or BCLs

Get Started

To get started with our Walk-Up Sequencing service for customer-prepared libraries, please fill out one of the following forms and a project manager will contact you within 1 business day.

Do you know which sequencing technology and cycle count you need for your project? Click below to get your order started.

Do you know which sequencing technology and cycle count you need for your project? Click below to get your order started.

Do you need assistance with selecting a sequencing technology and cycle count for your project? Click below to request a consultation.

Do you need assistance with selecting a sequencing technology and cycle count for your project? Click below to request a consultation.

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