Long-Read RNA Isoform
Sequencing
Enable high-throughput, long-read sequencing of full-length cDNA libraries with the novel Multiplexed Arrays Isoform Sequencing (MAS-seq) library preparation approach, commercially offered by PacBio as Kinnex™ full-length RNA kit for isoform sequencing. The combined accuracy and throughput enabled by Kinnex/MAS-seq greatly enhances the identification and quantification of RNA isoforms.
Long-Read RNA Isoform Sequencing with Broad Clinical Labs
Full-length RNA sequencing using long-read technologies is able to capture complete transcript isoforms, but this capability has been constrained by throughput limitations. Kinnex/MAS-seq is a technique that boosts throughput by a factor of 8x by programmatically concatenating cDNAs into single molecules optimal for long-read sequencing.
Kinnex 8x read output boost for bulk RNA-seq
The Kinnex protocol boosts long-read RNA throughput by ~8-fold from total RNA for a total output of 34–80M reads per 25M SMRTcell. Twelve samples are able to be multiplexed per Kinnex array.
Full-length RNA isoform ID
Full-length RNA sequencing enables robust isoform identification.
Accurate Transcript Fusion ID
Full-length RNA sequencing also enables accurate identification of transcript fusions and fusion isoforms.
Service Overview- Long-read RNA Isoform Sequencing (MAS-seq)
| Input Requirements (Total RNA) | Outputs |
|---|---|
| > 45 ng/µl > 30 µl > 7.0 RQS | 30–50 million HiFi DNA sequences per array in a de-concatenated, de-multiplexed, aggregated BAM file; QC metrics |
| Input Requirements (Total RNA) |
| > 45 ng/µl > 30 µl > 7.0 RQS |
| Outputs |
| 30–50 million HiFi DNA sequences per array in a de-concatenated, de-multiplexed, aggregated BAM file; QC metrics |
Project Workflow- Long-read RNA Isoform Sequencing
Project onboarding
(contracting if required)
Regulatory Review
Quoting
Sample Kits
Sample intake and QC
(extraction optional)
Sample receipt and incoming QC
Library construction
Iso-Seq express 2.0 kit
Kinnex full-length RNA kit
Sequencing
Pacific Biosciences
Revio™
Data and report delivery
(bioinformatic analysis optional)
See table above for deliverables (outputs)
Data delivered via secure Cloud based platform
To get started with Kinnex/MAS-seq services, please complete the form below, and a project manager will reach out to you.
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