Sequencing of Customer-Prepared Libraries

Receive publication-ready data quickly with rapid pre-prepared library sequencing services.

Start Your Sequencing Project with Our "Walk-Up" Service

Boston's Premier Genomics Partner for Rapid Sequencing Solutions

Broad Clinical Labs’ Sequencing of Customer-Prepared Libraries service delivers reliable results through expert handling of complex libraries—including unique adapters, low base diversity, and custom primers—and backed by the quality your research demands.

Have your ready-to-sequence libraries on a sequencer in as little as 2 days.

Our Technology Portfolio

NovaSeq X Plus starting as low as $2,375 per 25B lane

Illumina® NovaSeq X Plus

PacBio Revio™ long reads starting as low as $2,600 per SMRTcell ™

PacBio Revio™

Ultima Genomics UG 100™ starting as low as $4,000 per wafer

Ultima Genomics UG 100™

Additional Sequencers Available

Illumina® MiSeq

Illumina® NextSeq 2000

Illumina® NovaSeq 6000

Flexible, Scalable Solutions for your Genomic Studies

Accelerate discoveries across genomics, transcriptomics, and epigenomics research with our versatile high-quality sequencing solutions. From single lanes to full flowcells, we provide the flexibility you need to drive your research forward.

Have questions about the best sequencing approach for your research?

Talk to Our Specialists

Sequencing of Customer-Prepared Libraries with Broad Clinical Labs

Whats included: Sample kit for library submission, Library QC, sequencing prep, sequencing, and data delivery.

Service or tier/type Data Deliverables Minimum Reads

Illumina® NovaSeq X Plus 25B Single Lane*
Lowest price per Gb of data available!

*We enable ordering of individual NovaSeq X Plus lanes for paired end, dual-indexed libraries whose read structure follows these guidelines: read 1 ≤ 151 base pairs, index 1 ≤ 10 base pairs, index 2 ≤ 10 base pairs, read 2 ≤ 151 base pairs.
  • De-multiplexed FASTQ
  • ≥2.8 billion reads

Illumina® NovaSeq X Plus 25B/10B Flowcell
  • De-multiplexed FASTQ or BCL
  • 25B: ≥22 billion reads
  • 10B: ≥6 billion reads

Illumina® NovaSeq 6000 S4/S2/S1/SP Flowcell
  • De-multiplexed FASTQ or BCL
  • S4: ≥6 billion reads
  • S2: ≥3 billion reads
  • S1: ≥1 billion reads
  • SP: ≥500 million reads

Illumina® NextSeq 2000 P3/P2 Flowcell
  • De-multiplexed FASTQ or BCL
  • P3: ≥800 million reads
  • P2: ≥320 million reads

Illumina® MiSeq Flowcell
  • De-multiplexed FASTQ or BCL
  • ≥5 million reads

PacBio Revio™ Flowcell
  • HiFi Unaligned BAM
  • ≥4 million reads

Ultima Genomics UG 100™ Flowcell
  • CRAM
  • ≥8 billion reads

Scientific Excellence for Pre-Made Library Sequencing

Rapid, Cost-Effective Partnership

Libraries loaded onto sequencers in as little as two business days of receipt, at all-inclusive competitive pricing. Locals in the Boston area can also eliminate shipping costs and delays through walk-up service options.

Multi-Platform Flexibility with Scalable Solutions

Access a full portfolio of cutting-edge sequencing platforms, each with optimized workflows tailored for your research needs. Leverage advanced high-throughput sequencing infrastructure capable of seamlessly scaling as your project evolves.

Expert Handling and Publication-Ready Data

Leverage our 30+ years of sequencing expertise and CLIA-licensed, CAP-accredited facility standards with expert handling of even the most challenging library constructs. Comprehensive quality control protocols ensure reliable, publication-quality data.

Project Workflow- Sequencing of Customer-Prepared Libraries

Project onboarding

Project onboarding

Library Receipt at BCL

Library Receipt at BCL

Library QC

Library QC

Sequencing

Sequencing

Illumina® (short read)
PacBio (long read)
Ultima (short read)

Data delivery

Data delivery

Outputs:
Deliverables vary with sequencing technology, see table above

Project onboarding

Project onboarding

Library Receipt at BCL

Library Receipt at BCL

Library QC

Library QC

Sequencing

Sequencing

Illumina® (short read)
PacBio (long read)
Ultima (short read)

Data delivery

Data delivery

Outputs:
Deliverables vary with sequencing technology, see table above

Frequently Asked Questions

What are your minimum library concentrations?

Requirements vary by platform, typically ranging from a 2nM–5nM concentration. Contact our specialists to discuss specific requirements.

We sequence a wide variety of customer-prepared libraries including standard genomic DNA libraries, RNA-seq libraries, targeted panels, and complex constructs.

Yes! We offer flexible single-lane processing on the Illumina NovaSeq X Plus 25B. This provides cost-effective access to high-quality sequencing without requiring a full flowcell commitment. This service is only available for select read configurations—inquire about the service to speak to an Account Manager.

Data is delivered in a format compatible with customer-managed cloud-based storage, including Terra, Google Cloud Platforms, and Amazon Web Services. We also provide on-premises delivery for Broad associates.

Do you already know your preferred sequencing technology and read requirements? Click below to begin your order.

Ready to Order

Not sure which platform is right for your libraries? Connect with our team below to explore your options and initiate your project.

Consultation Request