RNA Sequencing

Unlock your transcriptome story with versatile, high-quality RNA sequencing for every sample type and research goal.

Begin Your RNA Sequencing Project

Advanced RNA Sequencing Solutions Made Accessible

Transform research limitations into breakthroughs with Broad Clinical Labs’ comprehensive RNA sequencing solutions. Our industry-leading expertise and cutting-edge technology overcome sample challenges to deliver high-quality results. Our innovative Total RNA Sequencing approach broadens access to whole transcriptome analysis, making comprehensive RNA research accessible across diverse fields through reduced costs, relaxed sample requirements, and automated workflows that capture the complete transcriptome beyond traditional mRNA sequencing.

From routine gene expression to challenging samples, we have the right solution for you.

Stranded mRNA

Traditional mRNA sequencing with poly(A) selection using workflows optimized for read depth and fragment insert size. Stringent quality controls and sample cleanup ensure accurate gene expression profiling & transcript discovery.

Total RNA

Increased sensitivity for low abundance transcripts through globin and rRNA depletion, enabling comprehensive transcriptome coverage that captures the full diversity of RNA species, including microRNA, circular RNAs, and long non-coding RNA.

Transcriptome Capture

Designed for low-input and degraded samples including FFPE tissues, this targeted hybridization capture approach leverages the TWIST Bioscience Alliance Clinical Research Panel, offering an enhanced alternative to traditional transcript enrichment methods.

Expert Extraction & Library Preparation

Precision begins before sequencing. Our automated extraction and library preparation workflows combine decades of optimization with state-of-the-art technology to maximize data quality from any sample type—from standard poly(A) selection to combined globin/rRNA depletion workflows—ensuring optimal library construction regardless of input quality or quantity. Our advanced features like unique molecular identifiers (UMIs) provide the technical precision that establishes Broad Clinical Labs as the industry leader in transcriptomics data quality.

Start Your RNA Sequencing Project

Transform Challenging Samples into Powerful Transcriptome Discoveries

Complete RNA Portfolio with Unmatched Flexibility

Access our suite of RNA sequencing services—Stranded mRNA, Total RNA, and Transcriptome Capture—offering the flexibility to evolve your research using one trusted provider.

Gold Standard Quality & Challenging Sample Expertise

Expert extraction and library preparation deliver high-quality, reproducible results from challenging sample types, including low-input and degraded specimens such as blood and formalin-fixed paraffin-embedded (FFPE) tissues.

Scalable Solutions and Advanced Technology

Leverage our high-throughput RNA analysis solutions that process 25,000+ whole transcriptomes annually, with advanced automated platforms, proven workflows, tailored data deliverables, and secure digital transfer optimized for your analysis needs.

Industry Leaders in Transcriptomics

Benefit from 30+ years of expertise and over 80,000 completed transcriptomes, backed by rigorous quality control measures and dedicated end-to-end specialist support.

Diverse Applications of RNA Sequencing

Discover the wide range of research our RNA sequencing services support.

Stranded mRNA

  • Differential gene expression
  • Isoform detection
  • Allele-specific mutation validation

Total RNA

  • Non-coding RNA detection & regulatory network analysis
  • Post-transcriptional modification studies
  • Alternative splicing analysis

Transcriptome Capture

  • FFPE tissue analysis for clinical & translational research
  • Analysis of low integrity samples
  • Cancer hotspot and targeted gene expression profiling

RNA Sequencing with Broad Clinical Labs

Service or tier/type Data Deliverables What’s Included

Stranded mRNA
50, 75, or 100 million reads aligned in pairs
  • CRAM file
  • Sample receipt and ERCC RNA control addition
  • Poly(A) selection and stranded cDNA synthesis
  • Sample fidelity QC
  • Sample qualification of matching DNA
  • Illumina sequencing
  • Data delivery

Total RNA
50, 100, or 200 million reads aligned in pairs
  • CRAM file (aligned to HG38)
  • Sample receipt
  • Combined globin & rRNA depletion, stranded cDNA synthesis, UMI enabled library construction
  • Illumina equencing on NovaSeqX
  • Data delivery

Transcriptome Capture
50 million reads aligned in pairs
  • STAR genome aligned BAM (HG19 or HG38)
  • STAR transcriptome aligned BAM (HG19 or HG38)
  • Sample fidelity QC upon request
  • Stranded cDNA synthesis, UMI enabled library construction
  • Targeted capture
  • Illumina sequencing
  • Data analysis, including alignment
  • Data delivery

Project Workflow- RNA Sequencing Service

Project onboarding

Project onboarding
(contracting if required)

Regulatory Review
Quoting
Sample Kits

Sample intake and QC

Sample intake and QC
(extraction optional)

Sample QC (including fingerprinting if DNA match sent)

Sample input requirements vary by RNA sequencing service

ERCC RNA control addition (Whole transcriptome)

Library Receipt at BCL

Library construction

Specialized protocol for each RNA sequencing service

Sequencing

Sequencing

Illumina NovaSeq X
Illumina NovaSeq 6000

Data delivery

Data and report delivery
(bioinformatic analysis optional)

Data delivered via secure cloud-based platform

See table above for data deliverables

Project onboarding

Project onboarding
(contracting if required)

Regulatory Review
Quoting
Sample Kits

Sample intake and QC

Sample intake and QC
(extraction optional)

Sample QC (including fingerprinting if DNA match sent)

Sample input requirements vary by RNA sequencing service

ERCC RNA control addition (Whole transcriptome)

Library Receipt at BCL

Library construction

Specialized protocol for each RNA sequencing service

Sequencing

Sequencing

Illumina NovaSeq X
Illumina NovaSeq 6000

Data delivery

Data and report delivery
(bioinformatic analysis optional)

Data delivered via secure cloud-based platform

See table above for data deliverables

Reach out to our team today to get started on your RNA sequencing project.

Start Your RNA Sequencing Project

Are you looking for long-read sequencing of full-length RNA?

Explore Our MAS-Seq Service